分类： 统计学 >> 应用统计数学 提交时间： 2019-04-22
摘要： Objective: The lifetime difference in adjacent parallel structure components becomes small as the number of components belonging to the same parallel structure increases. To infer the system structure, we must clarify the components that belong to the same parallel structure. Methods: A strengthened change point detection model (SCPDM) for weak mean difference data (WMDD) is established, which usually indicates that, as affected by a large variance, the mean difference in two subsignals for one data sequence becomes nonsignificant. For repeatedly retrievable WMDD, we performed two enhanced operations that doubled the mean difference by using the variance information and analyzed the asymptotic properties of the enhanced data. Then, we proposed an SCPDM based on the asymptotic results.Results: Finally, we compared the SCPDM with two other main change point detection models and verified that the SCPDM is superior to other models using WMDD change point detection by the simulation method.Limitations: This paper also have several limitations. First, we only discussed that are independent with normal distribution and single change point. Second, the reason why the relationship between and has an important influence on the accuracy of change point detection is not discussed in depth. We only defined the ratio boundary of WMDD by experience and simulation. Conclusions: Traditional change point detection models may become insensitive or ineffective for WMDD. We gave some asymptotic analysis and established a enhanced change point detection model (SCPDM) based on the asymptotic results. Compared with the traditional method, SCPDM can effectively detect the change point.
分类： 生物学 >> 生物物理学 提交时间： 2016-05-05
Wang, Xianlong
Cao, Chunwei
Huang, Jiaojiao
Yao, Jing
Hai, Tang
Zheng, Qiantao
Wang, Xiao
Zhang, Hongyong
Qin, Guosong
Zhou, Qi
Wang, Hongmei
Zhao, Jianguo
Zheng, Qiantao
Wang, Xiao
Zhang, Hongyong
Zhou, Qi
Wang, Hongmei
Zhao, Jianguo
Cheng, Jinbo
Yuan, Zengqiang
摘要： Pig shows multiple superior characteristics in anatomy, physiology, and genome that have made this species to be more suitable models for human diseases, especially for neurodegenerative diseases, because they have similar cerebral convolutions compared with human neocortex. Recently, CRISPR/Cas9 system shows enormous potential for engineering the pig genome. In this study, we expect to generate human Parkinson's disease pig model using CRISPR/Cas9 system by simultaneously targeting three distinct genomic loci, parkin/DJ-1/PINK1, in Bama miniature pigs. By co-injection of Cas9 mRNA and multiplexing single guide RNAs (sgRNAs) targeting parkin, DJ-1, and PINK1 genes, respectively, into in vivo derived pronuclear embryos, we simultaneously targeted three distinct genomic loci. The gene modified piglets remain healthy and display normal behavior at the age of 10 months. In addition, despite the high number of sgRNAs were employed in the present study, our trio-based whole-genome sequencing analysis suggested that the incidence of off-target events is low. Our results demonstrate that the simplicity, efficiency, and power of the CRISPR/Cas9 system to allow for the modification of multiple genes in pigs and yield results of high medical value.
点击量
待评议
点击量
下载量
评论
